- Acetate (carried from the Link reaction by coenzyme A) joins with Oxaloacetate (4C) to form Citrate (6C). CoA is the released to collect more acetate.
- Citrate is decarboxylated (CO2 is removed) and dehydrogenated (2H is removed) to form a 5C compound. 2H is accepted by a molecule NAD which becomes reduced NAD.
- 5C compound is also decarboxylated and dehydrogenated to form a 4C compound and a molecule of reduced NAD.
- 4C compound is changed to another 4C compound and during this change a molecule of ADP is phosphorylated to produce a molecule of ATP. (Substrate-level phosphorylation).
- The 4C compound then changes into another 4C compound and 2H is removed and accepted by coenzyme FAD which becomes reduced.
- The other 4C compound go through dehydrogenation and oxaloacetate is regenerated and another molecule of NAD is reduced.
There needs to be two turns round the cycle for each molecule of ATP
To conclude:
- 6 Reduced NAD are produced
- 2 Reduced FAD are produced
- 4 molecules of CO2 are produced
- and 2 ATP are produced
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